Loss of deoxyribonucleic acid-thymine during thymine starvation of Escherichia coli.

نویسندگان

  • T R Breitman
  • P B Maury
  • J N Toal
چکیده

A substantial loss of deoxyribonucleic acid-thymine occurs during thymine starvation of several thymine auxotrophs derived from Escherichia coli strains B, 15, and K-12.

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Thymine Starvation and Single-Strand Breaks in Chromosomal Deoxyribonucleic acid of Escherichia coli.

Single-strand breaks, as measured by the McGrath and Williams procedure, occur in chromosomal deoxyribonucleic acid of Escherichia coli cells during thymine starvation.

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Alkaline sucrose gradient sedimentation of chromosomal deoxyribonucleic acid from Escherichia coli PolA + and PolA - strains during thymine starvation.

Single-strand breaks were not detected in the deoxyribonucleic acid of Escherichia coli after thymine starvation for up to 180 min, even in a sensitive PolA(-) strain.

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Effect of thymine starvation on messenger ribonucleic acid synthesis in Escherichia coli.

Luzzati, Denise (Institut de Biologie Physico-Chimique, Paris, France). Effect of thymine starvation on messenger ribonucleic acid synthesis in Escherichia coli. J. Bacteriol. 92:1435-1446. 1966.-During the course of thymine starvation, the rate of synthesis of messenger ribonucleic acid (mRNA, the rapidly labeled fraction of the RNA which decays in the presence of dinitrophenol or which hybrid...

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Influence of thymine starvation on the integrity of deoxyribonucleic acid in Escherichia coli.

The influence of thymine starvation on the single-strand molecular weight of deoxyribonucleic acid (DNA) from Escherichia coli was determined by sedimentation through gradients of alkaline sucrose. Growth of cells for as long as 150 min in thymineless medium did not significantly reduce the molecular weight below the control value of 2.4 +/- 0.3 x 10(8) daltons. Incubation of cells in thyminele...

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Influence of starvation for methionine and other amino acids on subsequent bacterial deoxyribonucleic acid replication.

Billen, Daniel (University of Texas M. D. Anderson Hospital and Tumor Institute, Houston, Tex.), and Roger Hewitt. Influence of starvation for methionine and other amino acids on subsequent bacterial deoxyribonucleic acid replication. J. Bacteriol. 92:609-617. 1966.-A study has been made of the subsequent replicative fate of deoxyribonucleic acid (DNA) synthesized during amino acid starvation b...

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عنوان ژورنال:
  • Journal of bacteriology

دوره 112 1  شماره 

صفحات  -

تاریخ انتشار 1972